Morphophysiology and cryopreservation of seeds of Dendrobium nobile Lindl. (Orchidaceae) at different stages of development

The immature seeds of Dendrobium nobile acquire resistance to freezing and long-term storage in a cryobank at the age 6 months after pollination. Seed maturation Lindl establish optimal stage for liquid nitrogen was studied. Immature can germinate vitro starting from 3 pollination (MAP) but only develop up embryo swelling or protocorm without rhizoids. maximum staining embryos with vital dyes FDA TTC occurs 4 5 MAP that corresponds release suspensor beyond sac. Embryo does not correlate germination capacity seed viability cryogenic storage. drying airflow cryostorage MAP. 11% germinated MAP, 81–94% 7–10